Plant viral suppressors of post-transcriptional silencing do not suppress transcriptional silencing
نویسندگان
چکیده
منابع مشابه
Plant viral suppressors of RNA silencing.
RNA silencing is an ancient eukaryotic pathway in which double stranded RNA (dsRNA) triggers destruction of related RNAs in the cell. Early studies in plants pointed to a role for this pathway as a defense against viruses. Most known plant viruses have RNA genomes and replicate via dsRNA intermediates, thereby serving as potent inducers of RNA silencing early in replication and as silencing tar...
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Post-transcriptional gene silencing (PTGS) in plants results from the action of a mechanism that surveys the RNAs in a cell and specifically degrades those it perceives as alien. The alien RNAs can be doublestranded (ds) or single-stranded (ss) molecules that have homology to dsRNA present in the cell. Most plant viruses have RNA genomes that replicate to produce plus and minus sense RNAs, with...
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Virus-induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA-mediated gene silencing. There are a number of viruses which have been modified to silence the gene of interest effectively with a sequence-specific manner. Therefore, different types of methodologies ha...
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With the ongoing task of identifying and characterizing genes in the human genome, there is a great demand for tools to study these genes. Today, reverse genetics is the most effective way to explore gene function. This involves suppression of the gene of interest by a set of manipulation techniques. In addition to this, many conditions like viral infections, cancers and cardio-vascular disease...
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Post-transcriptional gene silencing (PTGS) is a homology-dependent RNA degradation process that may target RNA exclusively in the cytoplasm. In plants, PTGS functions as a natural defense mechanism against viruses. We reported previously that the 2b protein encoded by cucumber mosaic cucumovirus (CMV) is a virulence determinant and a suppressor of PTGS initiation in transgenic Nicotiana bentham...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 2000
ISSN: 0960-7412,1365-313X
DOI: 10.1046/j.1365-313x.2000.00710.x